Georgia (NIA VARDALOS) is an American professor of classical history who came to teach at Athens University in Greece. But due to cutbacks she has been forced to take a job as a tour guide working for Maria (BERNICE STEGERS) who gives the best tourists to Georgia's competitive coworker, Nico (ALISTAIR McGOWAN), who knows how to appease the wants and needs of his group.
That's unlike Georgia who wants her tourists to love Greek history and the locales she takes them to as much as she does. That's a losing battle, pointed out to her by her group's "class clown," Irv (RICHARD DREYFUSS), who also realizes that their bus driver, Poupi (ALEXIS GEORGOULIS), has a thing for her.
Among those traveling with them are American tourists, Kim (RACHEL DRATCH) and Big Al (HARLAND WILLIAMS) who are hoping to take advantage of her currently ovulating, while senior citizen couple Dorcas (SHEILA BERNETTE) and Barnaby (RALPH NOSSEK) are no longer focused on that, especially with Dorcas being a habitual thief.
Spanish divorcees Lena (MARIA ADANEZ) and Lala (MARIA BOTTO) are there looking for a good time, just like college student Gator (JAREB DAUPLAISE). Pancake house executive Marc (BRIAN PALERMO) is always connected back to the office, while teenager Caitlin (SOPHIE STUCKEY) isn't pleased to be traveling with her quarreling parents (CAROLINE GOODALL & IAN OGILVY).
With Nico making her look bad in comparison with his group, Georgia is frustrated with hers and her life in general. But with help from Irv and ultimately the others, she finds hope for a change for the better, especially in regard to her budding relationship with Poupi.