Two years after giving up their fowl-stealing ways, Mr. Fox (voice of GEORGE CLOONEY) and Mrs. Fox (voice of MERYL STREEP) are proud vulpine parents to Ash (voice of JASON SCHWARTZMAN) a brooding adolescent. Mr. Fox works as a columnist for the local paper and, ignoring the advice of his attorney, Badger (voice of BILL MURRAY), moves his family out of their standard hole in the ground and into the base of a large tree that comes along with its own superintendent, Kylie (voice of WALLY WOLODARSKY) the possum.
They're soon joined by Ash's cousin, Kristofferson (voice of ERICE ANDERSON), whose father is in the hospital, and Ash isn't happy about his own unfavorable comparisons to his relative. But there are bigger fish to fry in that Mr. Fox simply can't shake his animal instincts and the absolute joy he gets out of hunting fowl. Accordingly, he convinces Kylie and Kristofferson -- rather than Ash -- to join him in raiding the farms of three local human farmers, Walter Boggis (voice of ROBIN HURLSTONE), Nathan Bunce (voice of HUGO GUINNESS) and Franklin Bean (voice of MICHAEL GAMBON).
The latter produces strong alcoholic cider, employs Rat (voice of WILLEM DAFOE) to protect his goods, and is decidedly less than pleased that Mr. Fox and his companions have raided his stash, a feeling shared by Mrs. Fox who isn't happy that her husband has returned to his old ways. While that creates strife between the couple, they, those in their home, Badger and other local animals must contend with Bean convincing his fellow farmers to stop at nothing to find and kill the animals who've wronged them.