Sydney White (AMANDA BYNES) is a self-acknowledged tomboy who enjoys hanging out with the guys at construction sites where her plumber dad, Paul (JOHN SCHNEIDER), works. Yet, she's been accepted to Southern Atlantic University, her late mother's alma mater, and thus sets off for college life and the hope that she can join her mom's old sorority, Kappa Phi Nu. Unfortunately for her, it's run by Rachel Witchburn (SARA PAXTON), a vain and condescending young woman who uses the likes of sorority sisters Christy (LIBBY MINTZ) and Katy (LAUREEN LEECH) to do her dirty work. Moreover, and despite Sydney being a legacy, Rachel doesn't want her to join her exclusive Greek club.
Others at the school are more accepting of her, including her spunky roommate Dinky (CRYSTAL HUNT), who also pledges Kappa Phi, while Tyler Prince (MATT LONG) is a nice frat boy who takes a liking to Sydney. That doesn't sit well with Rachel who thinks of herself as Tyler's girlfriend, and thus she banishes Sydney from the sorority. With nowhere else to go, she ends up bunking in a rundown shack known as the Vortex. Among the students there is Lenny (JACK CARPENTER), a hypochondriac she earlier met and ditched as part of her sorority pledge requirements, as well as Spanky (SAMM LEVINE), a self-proclaimed but less than successful ladies man.
Also living there is brainiac Terrance (JEREMY HOWARD), the always-grumpy Gurkin (DANNY STRONG), the dopey George (ARNIE PANTOJA), bashful Jeremy (ADAM HENDERSHOTT) who always talks through his hand puppet, and the perpetually sleepy foreign exchange student Embele (DONT BONNER). As they try to help her look and act more like a girl -- in terms of winning over Tyler -- she sets out to help them upon learning that Rachel plans to have the Vortex torn down and replaced with a Greek center.