Buck Williams (KIRK CAMERON) is a young TV reporter who's interviewing scientist/researcher Chaim Rosenzweig (COLIN FOX) in Jerusalem about a breakthrough he's made in feeding the world's population. A sudden military attack on Israel interrupts them, but suddenly day turns into night and the attacking planes begin exploding and falling from the sky without ever being fired upon.
Meanwhile in Chicago, airline pilot Rayford Steele (BRAD JOHNSON) has just received a call that he has to head out early for a flight to London, much to the disapproval of his wife Irene (CHRISTINE MacFADYEN). It seems that their marriage is rather rocky, and she's upset that he'll miss the birthday party for his son, Raymie (JACK MANCHESTER), as will their 20-year-old daughter, Chloe (JANAYA STEPHENS), who's headed back to school.
Back in New York, Buck meets with Dirk Burton (JACK LANGEDIJK), a paranoid informant who tells him that some unscrupulous investment bankers, Johnathan Stonegal (DANIEL PILON) and Joshua Cothran (TONY DE SANTIS), are behind Rosenzweig's invention and are somehow involved with Nicolae Carpathia (GORDON CURRIE), an up and coming United Nations official.
Despite his wife's objections, Rayford sets out for his transatlantic flight with flight attendant Hattie Durham (CHELSEA NOBLE), who he's apparently having or is ready to have an affair with. Also on board is Kirk who also knows Hattie and has helped get her a job in the United Nations, with this being her last day in the air. Before she can celebrate, however, panic strikes the airliner when various people suddenly vanish in mid-flight, leaving only a pile of their clothing behind.
Rayford returns the flight to Chicago only to learn that millions of people have disappeared around the world, including his wife and son, and mass panic has broken out. While Rayford and Chloe console each other and find religion with the aid of Pastor Bruce Barnes (CLARENCE GILYARD) who briefly loses but then regains his own faith after this cataclysmic event, Buck sets out to figure out what's going on, eventually leading him to an encounter with none other than the Antichrist who's determined to rule the world.